Interstate 265 was a short connector between I-40 and the overlap of I-24/65 around the north side of Downtown Nashville.
Interstate 265 in Tennessee was decommissioned on April 7, 2000, per an action by the Route Numbering subcommittee of AASHTO. The route was renumbered as part of I-65 in an effort to better disperse through traffic around central Nashville. I-65 formerly overlapped with I-24 to the east of Downtown and with I-40 to the southeast. The reroute overtook I-265 to the north, and formed a new overlap with I-40 around the west side of the central business district.